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This data was then used to investigate phylogenetic relationships between these species based on complete cp DNA sequences, to estimate their times of divergence and to describe polymorphic cp DNA regions which will be useful for genetic analysis of cv. Genomic libraries were prepared according to manufacturer’s instructions (Illumina, San Diego, CA, USA).

This tree enabled us to calculate the most probable sequences of the protein titin of four common ancestors of the taxonomic groups to which these animals belong: placental mammals, dating back about 100 million years; all mammals, dating back 170-180 million years; the common ancestor of the sauropsids, including all birds, reptiles and also dinosaurs, and which lived about 280 million years ago; and the common ancestor of all the animals we have studied, which would be the common ancestor of the tetrapods, dating back about 350 million years," said Pérez-Jiménez.

Chloroplast SSRs usually show polymorphism, and are generally composed of poly-A or poly-T sequences of approximately 20 bp [ is available on public databases, complete chloroplast genome sequences for members of these genera have not been published to date.

Whole cp DNA sequence analysis could be used to investigate the phylogenetic relationships of the four cultivated species, identify chloroplast DNA markers (SSRs, SNPs and In Dels), estimate their time of divergence and contribute to the current understanding of the used as tropical grass forages.

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